Technology and Resources for Core Laboratories

The PRCTRC Technologies and Resources for Core Laboratories function provides a network of laboratory resources to support clinical and translational science in Puerto Rico. It provides access to expertise and instrumentation in available technologies for clinical and translational investigators. The following site contains information about the Bioanalytical Core Lab at the PRCTRC (MSC-UPR), Genomic Translational Research Unit (MSC-UPR), PHSU and UCC Services and Equipment Units.

  • Provide and develop training opportunities in emerging methodologies that are relevant to the clinical and translational investigators, technical and support staff.
  • Expand and maintain collaborations with public, private, and government organizations to increase access to additional research resources for clinical and translational investigators addressing health disparities.
  • Coordinate and provide continuous access of clinical and translational research laboratory services among PRCTRC.
  • Maintain and expand the Genomic Translational Research Unit.
  • In collaboration with PDC, provide training opportunities focusing on creating expertise in new and emerging technologies.
  • Establish the Research Training Opportunity Program (RTOP) to support emerging and innovative technologies.
  • In collaboration with CPC, maintain and expand the TRCL Network in Puerto Rico.
  • In collaboration with CPC and CBR, seek to make new alliances with core facility directors in Puerto Rico and elsewhere to provide greater access to research resources among PRCTRC investigators.
  • Collaborate with AC to implement the new charge-back policy to recover a percentage of the cost invested in federally funded studies.

About TRCL

Bioanalytical Core Lab at the PRCTRC (UPR-MSC)

Nurse

(Back) Carola Lopez-Cepero Román, Denisse Melecio, Nilda González
(Front) José Rodríguez-Medina

  • Vitros 250 (Routine Chemistry)
  • Hood
  • Microscope
  • Incubators
  • Refrigerators
  • Freezers
  • Water Baths
  • Stirrer Plates
  • Nano Drop 1000 Spectrophotometer
  • Clinitek 500 (Urinalysis)
  • DCA 2000+ (HB A1C)
  • Refrigerated Centrifuges
  • Micro-centrifuge
  • AcT 5diff  CP (Hematology)
  • Process Drugs in Urine
  • Centrifuge
  • Prepare slides
  • Storage (room temperature, refrigerated, frozen)
  • Packing
  • Shipping (room temperature, dry ice)
  • Preservation of samples (with diluents, buffers, etc.)
  • Process serum samples for Comprehensive Metabolic Panel (Glucose, BUN, Creatinine, electrolytes, etc.)
  • Process serum samples for Basic Metabolic Panel
  • Process serum samples for Lipid Panel
  • Process whole blood samples for HbA1c
  • Process whole blood samples for CBC (Complete Blood Count) and 5-part Differential
  • Process urine samples for Urinalysis
  • Process urine samples for Microalbumin
  • Process Pregnancy Tests
  • Isolation of PBMC from whole blood

PRCTRC Genomic Translational Research Unit (UPR-MSC)

The PRCTRC Genomic Translational Research Unit is housed in the second floor of the UPR-MSC main building, Rm. A231A (~250 square foot laboratory). The target faculty are investigators conducting genomics research at University Pediatric Hospital Newborn Screening Program, basic and clinical faculty conducting research in cancer diagnostics and cancer treatment at the UPR Comprehensive Cancer Center (UPRCCC), and UPR-MSC faculty conducting fundamental research in genomics, genetics and pharmacogenomics. Translational researchers working with model systems who have established collaborations with clinical faculty are also welcome.

Nurse

Lab Technician

  • DNA extraction and QC
  • RNA extraction, cDNA template preparation, and QC
  • Library Preparation for Sequencing, Templates preparation
  • DNA Sequencing Reactions (type of service: whole genome, whole exome, transcriptome (RNAseq), ChIPSeq, other)
  • Processing of raw data for subsequent bioinformatics analyses by investigator

Molecular Biology Core Laboratory (PHSU)

Nurse

Eileen Pabón, Pedro Santiago, Yashira García.

  • Gel and Blot Imaging Systems
  • Typhoon 7000 FLA, GE
  • Epi Chemi II Darkroom, UVP
  • Thermal Cycler and Q-PCR Systems
  • DNA Engine, BIO-RAD
  • DNA Engine DYAD, MJ Research
  • iCycler, BIO-RAD
  • Realplex2, Eppendorf
  • Eco, Illumina
  • Nucleic Acid and Protein
  • Quantification and Analysis
  • Biophotometer, Eppendorf
  • NanoDrop 2000, Thermo Sci.
  • Experion, BIO-RAD
  • Microscope and Cell Analysis
  • Cellometer K2, Nexcelom
  • Evos Fl, Life Technologies
  • BX60 microscope, Olympus
  • Multimode Plate Reader
  • Synergy HT, BioTek
  • Tissue and Sample Dissociators
  • gentleMACS Disruptor, Miltenyi
  • Bullet Blender Storm, Next Advance
  • Sonicator, Fisher
  • Tissue Culture
  • Transfection/Transformation
  • DNA, RNA and Protein Isolation
  • Cell Cryostorage
  • Expertise/consultation in experimental design using molecular biology

HIV and Substance of Abuse Laboratory Core (UCC)

Ivonne Amill, Zairaliz Reyes y Dr. Eddy Rios Oliveras

Zahiraliz Reyes, Ivonne Amill, and Dr. Eddy Rios Oliveras

  • FACSCalibur Flow Cytometer
  • Tabletop
  • Class II safety Cabinets
  • Refrigerators
  • Thermocycles
  • Sinks with foot pedal and eye-washer
  • Detection System
  • Repository for Lynphocytes and Plasma
  • Microarray Reader
  • Centrifuges
  • CO2-incubators
  • Ultrafreezers and FreezerWork
  • Monitor System
  • iCycler iQ Real-Time PCR
  • Cellometer Microplate Reader
  • Cellometer Microplate Reader
  • Elispot (pending)
  • HIV and HCV Genotyping System
  • The HIV and Substance of Abuse Laboratory Core (H-SALC) provides technical service needed to support studies in the areas of HIV/AIDS and related diseases (HCV, HBV) as well as in substances of abuse testing.