The PRCTRC Technologies and Resources for Core Laboratories function provides a network of laboratory resources to support clinical and translational science in Puerto Rico. It provides access to expertise and instrumentation in available technologies for clinical and translational investigators. The following site contains information about the Bioanalytical Core Lab at the PRCTRC (MSC-UPR), Genomic Translational Research Unit (MSC-UPR), PHSU and UCC Services and Equipment Units.

  • Provide and develop training opportunities in emerging methodologies that are relevant to the clinical and translational investigators, technical and support staff.
  • Expand and maintain collaborations with public, private, and government organizations to increase access to additional research resources for clinical and translational investigators addressing health disparities.
  • Coordinate and provide continuous access of clinical and translational research laboratory services among PRCTRC.
  • Maintain and expand the Genomic Translational Research Unit.
  • In collaboration with PDC, provide training opportunities focusing on creating expertise in new and emerging technologies.
  • Establish the Research Training Opportunity Program (RTOP) to support emerging and innovative technologies.
  • In collaboration with CPC, maintain and expand the TRCL Network in Puerto Rico.
  • In collaboration with CPC and CBR, seek to make new alliances with core facility directors in Puerto Rico and elsewhere to provide greater access to research resources among PRCTRC investigators.
  • Collaborate with AC to implement the new charge-back policy to recover a percentage of the cost invested in federally funded studies.

Core Accomplishments:

  • Developed training opportunities in emerging methodologies that are relevant to the clinical and translational investigators, technical and support staff.
  • Establish the Research Training Opportunity Program (RTOP). Four investigators affiliated to PRCTRC institutions have been supported.
  • Provides technical expertise, instrumentation, and services, while prioritizing investigator access to TRCL facilities and services according to the research area, translational potential, and clinical application of the project.
  • Establish the cost recovery system among clinical and translational research supported by TRCL in collaboration with the Administrative Core.
  • Support the development of the Puerto Rico Omic Center (PROMIC).
  • 224 studies have been supported by TRCL.
  • 97 investigators and faculty have been supported by TRCL.
  • 29 grants were awarded by TRCL users, for a total of $9,970,804.


YearStudies SupportedInvestigators SupportedPeer-Reviewed PublicationsGrants Applications SubmittedGrants Funded



Our Services

TRCL Active Projects

December 2016 to May 2017
Approval_DatePI Last NamePI NameTitle of StudyPI InstitutionSponsorHealth Disparity AreaOther Area
42088AlvesJanaina M.Utility of LIF to protect against HIV-1 associated neurocognitive disordersUCCInvestigNeurosci
42772AndriankajaOelisoa M.Lipid Lowering Agents use in Periodontitis and Diabetes Study (LLIPDS)UPR-MSCNIHOtherPeriodontitis / Diabetes
42640AppleyardCarolineTargeting of the Brain-gut-flora axis using probiotics in endometriosisPHSUNIHNeurosciMicrobiome
40983Banchs-PierettiHector L.A Randomized, Double-Blind, Placebo-Controlled, Event-Driven Trial of Quarterly Subcutaneous Canakinumab in the Prevention of Recurrent Cardiovascular Events among Stable Post-Myocardial Infarction Patients with Elevated hsCRPUPR-MSCIndustryCardiova
39862Bonilla-FelixMelvin A.Oxidative Stress and Carotid Intima-Media Thickness as Potential Markers of Early Cardiovascular Disease in Hipanic Children with Type 1 Diabetes MellitusUPR-MSCNIHCardiova
42174BoukliNawalIdentification of Toll Like Receptors Agonist-induced PBMC and CD8 + T Cells Anti-HIV Mechanism (Pilot Project B)UCCNIHhiv
42328BoukliNawalEffects of N-Acetylcysteine Amide (NACA) on Methamphetamine and HIV-1 Induced Oxidative Stress in Astrocyte CellsUCCOtherhiv
42634Burgos-CalderonRafaelA Phase 3, Multicenter, Randomized, Double-Blind, Placebo-Controlled Study Evaluating the Safety and Efficacy of Roxadustat for the Treatment of Anemia in Chronic Kidney Disease Patients not on DialysisUPR-MSCIndustryOtherAnemia / Chronic Kidney Diseases
41716Burgos-CalderonRafaelA Randomized, Multicountry, Multicenter, Double-Blind, Parallel, Placebo-Controlled Study of the Effects of Atrasentan on Renal Outcomes in Subjects with Type 2 Diabetes and NephropathyUPR-MSCIndustryOtherNephrology
41159Buxo-MartinezCarmen J.Oral Clefts Malformations in Puerto Rican Children: Genetic Risk Factors and Maternal ExposuresUPR-MSCNIHOtherOral Health
42716Campos-RiveraMaribelProspective Assessment of Intrauterine Zika Exposure: An Integrated ApproachUPR-MSCNIHNeurosci
42179CaninoGlorisa J.Epigenetic Variations of Asthma in Puerto Rican ChildrenUPR-MSCNIHCardiovaAsthma
42346CaninoGlorisaChildhood Adversity and Cardiovvascular Health among Puerto Rican YouthUPR-MSCNIHCardiova
39296Cruz-CorreaMarcia R.Familial Colorectal Cancer in Puerto Rico: A Feasibility StudyUPR-MSCNIHCancer
39534Cruz-CorreaMarcia R.Use of Curcumin for Treatment of Intestinal Adenomas in Familial Adenomatosus PoliposisUPR-MSCNIHCancer
42849Cruz-CorreaMarcia R.MAY2015-05-01 Randomized, double-blind, placebo-controlled trial of Meriva (curcumin) as a candidate chemoprevention agent for gastric carcinogenesisUPR-MSCCancer
42863Cruz-CorreaMarcia R.MAY2013-02-01 Phase I Trial of a Therapeutic DNA Vaccine for Chronic Hepatitis C Virus (HCV) InfectionUPR-MSCNIHCancer
41831Cruz-CorreaMarcia R.Randomized, Double-Blind, Placebo-Controlled Trial of MUC1 Vaccine in Patients with Newly Diagnosed Advanced AdenomasUPR-MSCNIHCancer
42899Cruz-CorreaMarcia R.Targeted chemoprevention of gastric carcinogenesis in high risk populationsUPR-MSCNIHCancer
42718De Jesus-GonzalezNilkaGenome Wide ASsociation Study of Childhood Onset Idiopathic Nephrotic SyndromeUPR-MSCInvestigOtherPediatric Neprhro
42832Diaz-PerezClementeA Phase 2/2b, Randomized Trial to Evaluate the Safety, Immunogenicity and Efficacy of a Zika Virus DNA Vaccine in Healthy Adults and AdolescentsUPR-MSCNIHOtherVaccine Research
42745Diaz-PerezClementeA Multicenter Specimen Collection Protocol to Obtain Human Biological Samples for Research StudiesUPR-MSCNIHOtherInfectious Disease
42303Diaz-PerezClementePhase 2 Randomized, Placebo-Controlled Trial to Evaluate the Safety and Immunogenicity of a Chikungunya Virus-Like Particle Vaccine, VRC-CHKVLP059-00-VP, in Healthy AdultsUPR-MSCNIHOtherChikungunya Virus
41681Estape-GarrastazuEstela S.Assessment of Cardiotonic Steroids in Ischemic Stroke PatientsUPR-MSCNIHCardiova
42178Gerena-LopezYamilBiomarkers and Molecular Mechanisms Associated with Insulin Resistance in Patients with Type 1 and Type 2 DiabetesUPR-MSCInvestigCardiova
41876Gonzalez-MercadoVeldaAssociation between Differential Gene Expression and Fatigue Symptoms of Puerto Rican patients during Cancer TreatmentsUPR-MSCNIHCancer
42099GuiotHumbertoProtective Effect of Quadrivalent Vaccine in Young HIV-positive Males Who Have Sex with MalesUPR-MSCNIHCancer
42746Guiot-MartinezHumbertoA Randomized phase III study of intra-anal Imiquimod 2.5% vs Tropical-5 fluorouracil 5% vs Observation for the treatment of high-grade anal squamous intra epithelial lesions in HIV-infected men and womenUPR-MSCNIHhivCancer / HPV
40436Hunter-MelladoRobert F.Retrovirus Research Center Leadership CoreUCCNIHhiv
40301Irizarry-RamirezMargaritaIdentification of Prostate Cancer Stage in Hispanics using Serum Protein ProfileUPR-MSCNIHCancer
41869Jimenez-VelazquezIvonne Z.A Phase II/III Randomized, Double-Blind, Placebo-Controlled Multi-Center Study of 2 Potential Disease Modifying Therapies in Individuals at Risk for and With Dominantly Inherited Alzheimer's DiseaseUPR-MSCIndustryOtherGeriatrics
38730Jimenez-VelazquezIvonne Z.Risk Factors for Alzheimer's Disease and Related Disorders in the Caribbean Hispanic Population of Puerto RicoUPR-MSCNIHNeurosci
42627Jimenez-VelazquezIvonne Z.A 24-month, Multicenter, Randomized, Double-Blind, Placebo Controlled, Parallel-Group, Efficacy, Safety, Tolerability, Biomarker, and Pharmacokinetic Study of AZD3293 in Early Alzheimer's Disease (The AMARANTH Study)UPR-MSCIndustryNeurosci
42031Lopez-del-ValleLydiaGenetic and Environmental Factors of MalocckusionUPR-MSCNIHOtherDentistry
42328MartinezIdaliIdentification of biomarker of chronic arthralgia after Chikungunya infectionUPR-MSCNIHOtherInfectious Disease
41731Nazario-DelgadoCruz M.Red para la investigacion de salud en el Caribe Oriental-ECHORN [�Eastern Caribbean Health Outcome Research Network (ECHORN)�UPR-MSCNIHOtherChronic disease (Cardiovascular and Cancer)
41298Ortiz-Martinez de AndinoAna P.Detection of HPV Infection in the Oral Cavity of Hispanics with and without Periodontal DiseaseUPR-MSCNIHCancer
42639Palacios-AlzurusCristinaEffect of Soluble Corn Fiber supplementation for 1 year on bone metabolism in adolescents (1SC1HD089556-01)UPR-MSCNIHOtherbone
42703RiosGrisselPhase 3, Randomized, Double-Blind Study Comparing ABT-494 Once Daily Monotherapy to Methotrexate (MTX) Monotherapy in MTX-Naive Subjects with Moderately to Severely Active Rheumatoid ArthritisUPR-MSCIndustryOtherRheumatology
42192Rios-SolaGrisselAn Open-Label Extension Study of DS-5565 for 52 Weeks in Pain Associated with FibromyalgiaUPR-MSCIndustryOtherRheumatology
42509Rios-SolaGrisselA Phase III, Multicenter, Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Study to Assess the Efficacy and Safety of Tocilizumab versus Placebo in PAtients with Systemic SclerosisUPR-MSCIndustryOtherRheumatology
42621Rivera-AmillVanessaThe microbiome in the setting of HIV infection and DepressionPHSUInvestighiv
42621Rivera-AmillVanessaMetabolic syndrome and prophylactic anti-retrovirals in men who have sex with menPHSUNIHhiv
42809RomagueraJosefinaExtension Study to Evaluate the Efficacy and Safety of Elagolix in Premenopausal Women with Heavy Menstrual Bleeding Associated with Uterine FibroidsUPR-MSCIndustryOtherOB Gyn
41940Romaguera-AgraitJosefina E.An in-depth high-throughput study on the role of the cervicovaginal microbiome in HPV infectionsUPR-MSCNIHOtherObstetrics and Gynecology
42278Romaguera-AgraitJosefina E.A Phase 3, single arm, clinical trial to study the contraceptive efficacy and safety of the MK- 8342B (etonegestrel + 17β-estradiol) vaginal ring in healthy women 18 years of age and older, at risk for pregnancyUPR-MSCIndustryOtherObstetrics and Gynecology
42874Romaguera-AgraitJosefina E.A Prospective, Randomized, Double-Blind, Placebo-Controlled Phase 3 Study of VGX-3100 delivered Intramuscularly followed by Electroporation with Cellectra 5PSP for the Treatment of HPV-16 and/or HPV-18 related High Grade Squamous Intraepithelial Lesion (HSIL) of the Cervix (Version 3.0 23 Sep 2016)UPR-MSCIndustryOtherObstetrics and Gynecology
41731Romaguera-AgraitJosefina E.Pilot biomarker study to evaluate urine-based HPV-TrDNA testing as screening tool in healthy women and cervical cancer patientsUPR-MSCInvestigCancer
42467Romeguera-AgraitJosefinaA Phase 3 Study to Evaluate the Efficacy and Safety of Elagolix in Combination with Estradiol/Norethindrone Acetate for the Managementof Heavy Menstrual Bleeding Associated with Uterine Fibroids in Premenopausal WomenUPR-MSCIndustryOtherObstetrics and Gynecology
42603SaavedraHaroldAddressing novel roles of the TTK kinase in Puerto Rican breast cancer patientsPHSUOtherCancer
42046SabateNuriaClinical prevalence and biological correlates of PTSD in children and adolescents in southwest Puerto RicoPHSUNIHNeurosci
42745Serrano-RamosCarmenA randomized, double-blind, placebo-controlled trial of urate-elevating inosine treatment to slow clinical decline in early Parkinson's diseaseUPR-MSCNIHNeurosci
40589Suarez-RiveraMarta P.Cardiometabolic Risk Factors, Adipokines, Oxidative Stress, and Vitamin D: A Cross-Sectional Analysis of Junior and High School Students in Puerto RicoUPR-MSCNIHCardiova
41870TiradoMaribelScreening HIV Infected Women for Anal Cancer PrecursorsUPR-MSCNIHCancer
42320Tirado-GomezMaribelANCHOR: Anal Cancer Prevention StudyUPR-MSCNIHCancerHIV
42488TorresEsther A.Genotypic and phenotypic manifestations and disease progression in Puerto Ricans with Inflammatory Bowel Disease and Hermansky-Pudlak SyndromeUPR-MSCInvestigOther
41912Vila-PerezLuis M.Association of genetic and autoantibody signatures with SLE clinical courseUPR-MSCNIHOtherAutoimmune rheumatic diseases
42076Wojna-MunizValerieH024 Maraviroc and NeuroAIDS PathogenesisUPR-MSCNIHhiv
40878Wojna-MunizValerieSoluble Insulin Receptor Dysfuntion Correlates with HAND in HIV-Woman on CARTUPR-MSCNIHNeurosci
42290Wojna-MunizValerieExosome-mediated soluble insulin receptor secretion correlates with HANDUPR-MSCNeurosci
41186Wojna-MunizValerieInsulin Resistance and CSF Biomarkers in Puerto Ricans with MCI and Early Alzheimer's DiseaseUPR-MSCNIHNeurosci

(Back) Carola Lopez-Cepero Román, Denisse Melecio, Nilda González
(Front) José Rodríguez-Medina



  • Vitros 250 (Routine Chemistry)
  • Hood
  • Microscope
  • Incubators
  • Refrigerators
  • Freezers
  • Water Baths
  • Stirrer Plates
  • Clinitek 500 (Urinalysis)
  • DCA 2000+ (HbA1c and Microalbumin)
  • Refrigerated Centrifuges
  • Micro-centrifuge
  • AcT 5diff CP (Hematology)


  • Centrifuge
  • Prepare slides
  • Storage (room temperature, refrigerated, frozen)
  • Packing
  • Shipping (room temperature, dry ice)
  • Preservation of samples (with diluents, buffers, etc.)
  • Process serum samples for Comprehensive Metabolic Panel
  • Process serum samples for Basic Metabolic Panel
  • Process serum samples for Lipid Panel
  • Process whole blood samples for HbA1c
  • Process whole blood samples for CBC (Complete Blood Count) and 5-part Differential
  • Process urine samples for Urinalysis
  • Process urine samples for Microalbumin
  • Process urine samples for Pregnancy Test
  • Isolation of PBMC from whole blood (initial steps only:
    separation and aliquoting of plasma and buffy coat)

Lab Technician

The PRCTRC Genomic Translational Research Unit is housed in the second floor of the UPR-MSC main building, Rm. A231A (~250 square foot laboratory). The target faculty are investigators conducting genomics research at University Pediatric Hospital Newborn Screening Program, basic and clinical faculty conducting research in cancer diagnostics and cancer treatment at the UPR Comprehensive Cancer Center (UPRCCC), and UPR-MSC faculty conducting fundamental research in genomics, genetics and pharmacogenomics. Translational researchers working with model systems who have established collaborations with clinical faculty are also welcome.


  • DNA extraction and QC
  • RNA extraction, cDNA template preparation, and QC
  • Library Preparation for Sequencing, Templates preparation
  • DNA Sequencing Reactions (type of service: whole genome, whole exome, transcriptome (RNAseq), ChIPSeq, other)
  • Processing of raw data for subsequent bioinformatics analyses by investigator


  • DNA extraction and QC
  • RNA extraction, cDNA template preparation, and QC
  • Library Preparation for Sequencing, Templates preparation
  • DNA Sequencing Reactions (type of service: whole genome, whole exome, transcriptome (RNAseq), ChIPSeq, other)
  • Processing of raw data for subsequent bioinformatics analyses by investigator

Camille Chardón, Pedro Santiago and Jennifer Cabán.


  • Gel and Blot Imaging Systems
  • Typhoon 7000 FLA, GE
  • Epi Chemi II Darkroom, UVP
  • Thermal Cycler and Q-PCR Systems
  • DNA Engine, BIO-RAD
  • DNA Engine DYAD, MJ Research
  • iCycler, BIO-RAD
  • Realplex2, Eppendorf
  • Eco, Illumina
  • Nucleic Acid and Protein
  • Quantification and Analysis
  • Biophotometer, Eppendorf
  • NanoDrop 2000, Thermo Sci.
  • Experion, BIO-RAD
  • Microscope and Cell Analysis
  • Cellometer K2, Nexcelom
  • Evos Fl, Life Technologies
  • BX60 microscope, Olympus
  • Multimode Plate Reader
  • Synergy HT, BioTek
  • Tissue and Sample Dissociators
  • gentleMACS Disruptor, Miltenyi
  • Bullet Blender Storm, Next Advance
  • Sonicator, Fisher


  • Tissue Culture
  • Transfection/Transformation
  • DNA, RNA and Protein Isolation
  • Cell Cryostorage
  • Expertise/consultation in experimental design using molecular biology

Zahiraliz Reyes, Ivonne Amill, and Dr. Eddy Rios Olivares


  • FACSCanto II
  • Class II Safety Cabinets
  • Refrigerators
  • Thermocycles
  • Repository for Lymphocytes and Plasma
  • Centrifuges
  • CO2-incubators


The HIV and Substance of Abuse Laboratory Core (H-SALC) provides technical service needed to support studies in the areas of HIV/AIDS and related diseases.

  • Ultrafreezers
  • iCycler iQ Real-Time PCR
  • Elispot

Standard Operation Procedures

Dr. Pedro Santiago-Cardona (PHSU)
Co-Program Director
787-840-2575, ext. 2200 ó 2248

Camille M. Chardón Colón (PHSU), MS
Research Laboratory Technician
Tel: 787.840.2575 x2200

Jennifer Y. Cabán(PHSU), MPH
Research Laboratory Technician
Tel: 787.840.2575 x2200

Dr. Misty Eaton (UCC)
Key Function Co-Leader
787 798-3001 ext. 2034

Zahiraliz Reyes Bou(UCC)
Research Assistant
Tel. 787-798-3001 ext. 2079

Ivonne Amill, BA (UCC)
787 -798-3001
Ivonne.amill@uccaribe. edu

Dr. José Rodríguez Medina (MSC)
Key Function Leader -RCM
758-2525 ext 2299

Nilda L. González Hernández, Ms, MT(ASCP)  (MSC)
Core Laboratory Manager
787-759-0306 ext 226

Carola M. López-Cepero Román, Ms, MT (ASCP) (MSC)
Medical Technologist II
787-759-0306 ext 226
carola.lopez-cepero@upr. edu